Trypsin-free cell harvesting
Trypsin-free cell harvesting
Developed using a vegetable-based component rather than trypsin, the SplitKits system has been specifically designed for use in both serum-based and serum-free cell harvesting methodologies and is suitable for a wide variety of cell culture systems. With faster cell dissociation than trypsin, the extremely gentle action of the vegetable-based enzyme gives higher cell yields and better cell growth recovery following detachment. In addition, surface epitopes and cell membranes remain unaltered and fully functional, making the system ideal for use in flow cytometry. Greater control of the dissociation process produces homogeneous cell suspensions with minimal batch-to-batch variation. With no animal-based products involved, the system avoids the risk of contamination with either animal or human viruses or bacteria and offers the industry a potentially safer, more compliant system for cell culture. The kit is composed of the vegetable-based enzymatic reagent (SplitCells), AntiSplitCells and SplitCells buffer, resulting in a fully flexible cell dissociation system that can perform at ambient temperature. Three different formats are available and are optimised for use with specific cell types: SplitKit Strong, for strongly adherent and bioreactor-cultured cells; SplitKit Moderate for moderately adherent cells and SplitKit Mild for mildly adherent cells.
Sera Laboratories International Ltd
