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Products News Coupling of SPR imaging and mass spectrometry SPRi biochips SPR imaging (SPRi) allows for the real-time and label-free monitoring of multiplexed biomolecular interactions. Coupling SPRi to MALDI mass spectrometry permits the tracking and capture of analytes by SPR imaging and their identification by mass spectrometry. However, most methodologies require the elution of the bound analyte and its re-deposition on the MS plate. This procedure has many drawbacks, including analysis time, no multiplexing capabilities, decreased sensitivity and additional cross-contamination risks, which previously delayed the development of SPR-MS in the diagnostics field. The open format of HORIBA Scientific's SPRi biochips enables a single biochip to be used for both SPRi and MALDi- MS. The biochip used for SPRi (SPRi-Slide) is directly transferred to the MS instrument. There is no need to either elute or re-deposit the bound analyte. The MS enzymatic digestion and the deposition of the MALDI matrix are performed directly on the SPRi-Slide. The latter is then placed on the MS plate holder.This workflow has been successful with different MALDI-MS instruments on an antibody-protein interaction model, as well as with human plasma for ligand fishing. HORIBA Jobin Yvon S.A.S. Supplier details Download above as PDF Contact form Get in touch directly with the above supplier Pre-fill this form automatically in My BTI Last name:* Firstname:* Company/Organisation:* Job title:* City:* Postal Code:* Country:* Email:* Email (confirm):* Your email address will not be communicated to any third party other than the above supplier for the purpose of fulfilling this enquiry. For more information: BTI's privacy policy Tel: Message:     Send product data Send price data Send dealer data         I would like to receive BTI's electronic publications

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