Columns for accelerated LC/MS analysis of syntheti cligonucleotides
syntheti cligonucleotides
Developed for rapid and efficient LC/MS characterisation as well as quality control of synthetic RNA and DNA, the Clarity Oligo-MS, C18 columns – part of the Clarity BioSolutions portfolio from Phenomenex – deliver significantly shorter run times with increased resolution and sensitivity. These columns, packed with either 2.6µm or 1.7µm core-shell particles, enable high efficiency on any HPLC or UHPLC platform. The 2.6µm columns operate at reduced backpressures compared to other oligonucleotide-specific columns, delivering UHPLC performance on any LC instrument and methods are easily transferable. The 1.7µm columns boost the performance of existing sub-2µm methods on high-pressure systems. LC/MS is the analysis technique of choice in synthetic oligo quality control as well as for the characterisation of oligo-based therapeutics and their metabolites; however with traditional fully porous particles, efficiency decreases as flow rate increases, resulting in loss of resolution and sensitivity and longer overall analysis time. The core-shell technology of the C18 columns enables high resolution and sensitivity over an extended linear velocity without generating excessive backpressure and means that impurities in complex synthetic mixtures can be separated from the peak of interest in less than 10 minutes. This technology provides roughly twice the efficiency of fully porous 3-micron columns and three times the efficiency of fully porous 5-micron columns, with significantly lower limits of detection and quantitation.
Phenomenex
