MALDI-TOF Mass Spectrometry:
High-Throughput Proteomics Takes Flight
Many of the greatest discoveries in biology and medicine have been preceded by key technological developments. Recent advances in matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry have created several new possibilities for this technology and are opening new doors in large-scale biology and clinical diagnostics, in particular for applications in high-throughput proteomics. [More]
A vision of biomarker discovery using peptidomics
In the field of clinical diagnostics, there is increasing interest in native peptides as potentially useful biomarkers. As human proteins and peptides are biosynthesized, processed, and degraded, many structurally related peptide intermediates are produced. Thousands of such peptides are present in human body fluids and tissues and these can be profiled using reversed-phase chromatography in combination with MALDI-TOF mass spectrometry. [More]
Seeing SNPs in a new light
Single nucleotide polymorphisms (SNPs) are the most abundant genetic variations between individuals and are easily detected by several methods including MALDI-TOF spectrometry. An interesting application of SNPs beyond their obvious utility for normal genotyping is their role in allele- or haplotype-specific gene expression. [More]
